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human oscc cell line cal27 (ATCC)

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ATCC human oscc cell line cal27
Human Oscc Cell Line Cal27, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1953 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+oscc+cell+line+cal27/CAL+27/pm42265343-60-3-9
Average 98 stars, based on 1953 article reviews

human oscc cell line cal27 - by Bioz Stars, 2026-09

98/100 stars

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Article Title: Waterpipe Smoke-Induced N6-Methyladenosine Modification Promotes the Progression of Oral Squamous Cell Carcinoma
Article Snippet: .. The human OSCC cell line CAL27 was obtained from the American Type Culture Collection (ATCC, USA) and cultured in DMEM supplemented with 10% FBS and antibiotics. ..

Article Title: Waterpipe Smoke-Induced N6-Methyladenosine Modification Promotes the Progression of Oral Squamous Cell Carcinoma.
Article Snippet: .. Cell culture andWPSC treatment The human OSCC cell line CAL27 was obtained from the American Type Culture Collection (ATCC, USA) and cultured in DMEM supplemented with 10% FBS and antibiotics. .. WPSC was prepared as previously described and used to treat CAL27 cells at optimized concentrations for 48 hours.22,23 Untreated cells served as controls. mRNA expression analysis Total RNA was isolated using TRIzol reagent (Invitrogen, Carlsbad, CA, USA), and cDNA was synthesized with the PrimeScript 1st Strand cDNA Synthesis Kit (TaKaRa, Japan).

Article Title: FBXW7 Enhances Cisplatin-Induced Apoptosis in Oral Cancer Cell Lines
Article Snippet: .. Human OSCC cell line CAL27, SCC9 was acquired from American Type Culture Collection (ATCC) and was cultured in RPMI-1640 medium with 10% fetal bovine serum (Gibco) and 1% penicillin/streptomycin. ..

Article Title: Identification of epithelial, mesenchymal, and platelet-associated circulating tumour cells with translational implications in oral squamous cell carcinoma.
Article Snippet: .. Cell Culture: The human OSCC cell line Cal27 (CRL-2095TM, ATCC®) was used in spike-in experiments. .. Cells were maintained at 37°C, 5% CO2 in Dulbecco's Modified Eagle Medium (DMEM) (Lonza, #12-604F) supplemented with 10% Foetal Bovine Serum (FBS) (Gibco, #10270106) and Antibiotic-Antimycotic (Gibco, #15240062).

Article Title: Transcriptome analysis of key genes and pathways associated with cisplatin resistance in oral squamous cell carcinoma Cal27 cells.
Article Snippet: Background Oral squamous cell carcinoma (OSCC) has a poor postoperative recovery and is prone to drug resistance during long-term chemotherapy, but the molecular mechanism of its resistance has not been fully elucidated.. Methods In the present study, a cisplatin-resistant cell line Cal27R was established and the key genes and pathways associated with drug resistance were explored using bioinformatics analysis and molecular biology experimental techniques.. Results Transcriptome analysis reveals a total of 1927 differentially expressed genes (DEGs).

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Citotoxicity induced by CdCl 2 is reversed by the ferroptosis and autophagy inhibitors, Fer-1 and Baf, only in CAL27 cells. (A) Representative plots of Annexin V/7-AAD apoptosis assay (left) and relative histograms (right) of CAL27 and SCC154 cells upon treatment with 26.01μM CdCl 2 (12h). (B) PI flow cytometry assay and relative histograms of CAL27 and SCC154 cells treated with CdCl 2 (26.01μM for 12h) alone or in combination with Fer-1 (100μM for 24h) and Baf (1μM for 12h). % of dead cells (PI positive) are reported in each dot plot. All data represent the mean of three independent experiments. Histograms are reported as mean ± SD. p -value: *≤0.05; ****≤0.0001. ns: not significant.

Journal: International Journal of Biological Sciences

Article Title: Acute Exposure to Cadmium Triggers NCOA4-Mediated Ferritinophagy and Ferroptosis in Never-Smokers Oral Cancer Cells

doi: 10.7150/ijbs.111228

Figure Lengend Snippet: Citotoxicity induced by CdCl 2 is reversed by the ferroptosis and autophagy inhibitors, Fer-1 and Baf, only in CAL27 cells. (A) Representative plots of Annexin V/7-AAD apoptosis assay (left) and relative histograms (right) of CAL27 and SCC154 cells upon treatment with 26.01μM CdCl 2 (12h). (B) PI flow cytometry assay and relative histograms of CAL27 and SCC154 cells treated with CdCl 2 (26.01μM for 12h) alone or in combination with Fer-1 (100μM for 24h) and Baf (1μM for 12h). % of dead cells (PI positive) are reported in each dot plot. All data represent the mean of three independent experiments. Histograms are reported as mean ± SD. p -value: *≤0.05; ****≤0.0001. ns: not significant.

Article Snippet: Human oral squamous cell lines (OSCC) - CAL27, OT1109, SCC090, and SCC154 - were purchased from the American Type Culture Collection (ATCC, Rockville, MD, United States).

Techniques: Apoptosis Assay, Flow Cytometry

CdCl 2 administration induces NCOA4-mediated ferritinophagy in CAL27 cells. (A) Western blot analysis and relative optical densitometry of NCOA4, FtH1, LC3B (I-II) and mTORC1 and p-mTORC1 in CAL27 and SCC154 cells treated with 26.01μM CdCl 2 (12h). GAPDH was used as normalization control for protein quantification. (B) Representative images of morphological and ultrastructural features detected by TEM in CAL27 and SCC154 upon treatment with CdCl 2 (26.01μM for 12h). Yellow arrows, autophagosome. (C) Fluorescence microscopy analysis of LIP content with FerroOrange dye in CAL27 and SCC154 cells after treatment with 26.01μM CdCl 2 (12h). All the experiments were carried out in triplicate. Histograms are reported as mean ± SD. p -value: **≤0.01; ***≤0.001; ****≤0.0001. ns: not significant.

Journal: International Journal of Biological Sciences

Article Title: Acute Exposure to Cadmium Triggers NCOA4-Mediated Ferritinophagy and Ferroptosis in Never-Smokers Oral Cancer Cells

doi: 10.7150/ijbs.111228

Figure Lengend Snippet: CdCl 2 administration induces NCOA4-mediated ferritinophagy in CAL27 cells. (A) Western blot analysis and relative optical densitometry of NCOA4, FtH1, LC3B (I-II) and mTORC1 and p-mTORC1 in CAL27 and SCC154 cells treated with 26.01μM CdCl 2 (12h). GAPDH was used as normalization control for protein quantification. (B) Representative images of morphological and ultrastructural features detected by TEM in CAL27 and SCC154 upon treatment with CdCl 2 (26.01μM for 12h). Yellow arrows, autophagosome. (C) Fluorescence microscopy analysis of LIP content with FerroOrange dye in CAL27 and SCC154 cells after treatment with 26.01μM CdCl 2 (12h). All the experiments were carried out in triplicate. Histograms are reported as mean ± SD. p -value: **≤0.01; ***≤0.001; ****≤0.0001. ns: not significant.

Article Snippet: Human oral squamous cell lines (OSCC) - CAL27, OT1109, SCC090, and SCC154 - were purchased from the American Type Culture Collection (ATCC, Rockville, MD, United States).

Techniques: Western Blot, Control, Fluorescence, Microscopy

CdCl 2 treatment triggers mitochondrial dysfunction only in CAL27 cells. Flow cytometry analyses and relative histograms of mitochondrial ROS levels (A) and mitochondrial membrane potential (B) assessed by MitoSOX and TMRM reagents, respectively, in CAL27 and SCC154 cells following treatment with 26.01μM CdCl 2 (12h). (C) Representative images of morphological and ultrastructural features detected by TEM in CAL27 and SCC154 upon treatment with CdCl 2 (26.01μM for 12h). Pink arrows: damaged mitochondria. Each experiment was performed in triplicate. Histograms are presented as mean ± SD. p -value: ***≤0.001; ****≤0.0001. ns: not significant.

Journal: International Journal of Biological Sciences

Article Title: Acute Exposure to Cadmium Triggers NCOA4-Mediated Ferritinophagy and Ferroptosis in Never-Smokers Oral Cancer Cells

doi: 10.7150/ijbs.111228

Figure Lengend Snippet: CdCl 2 treatment triggers mitochondrial dysfunction only in CAL27 cells. Flow cytometry analyses and relative histograms of mitochondrial ROS levels (A) and mitochondrial membrane potential (B) assessed by MitoSOX and TMRM reagents, respectively, in CAL27 and SCC154 cells following treatment with 26.01μM CdCl 2 (12h). (C) Representative images of morphological and ultrastructural features detected by TEM in CAL27 and SCC154 upon treatment with CdCl 2 (26.01μM for 12h). Pink arrows: damaged mitochondria. Each experiment was performed in triplicate. Histograms are presented as mean ± SD. p -value: ***≤0.001; ****≤0.0001. ns: not significant.

Article Snippet: Human oral squamous cell lines (OSCC) - CAL27, OT1109, SCC090, and SCC154 - were purchased from the American Type Culture Collection (ATCC, Rockville, MD, United States).

Techniques: Flow Cytometry, Membrane

CAL27 cells shows lipid peroxidation after CdCl 2 administration. (A) Flow cytometry analysis and relative histograms of lipid peroxidation quantified by using BODIPY-C11 in CAL27 and SCC154 cells upon treatment with 26.01μM CdCl 2 (12h). (B) Western blot analysis and relative optical densitometry of GPX4 in CAL27 and SCC154 cells treated with 26.01μM CdCl 2 (12h). GAPDH was used as normalization control for protein quantification. (C) Fluorescence microscopy analysis of GSH content in CAL27 and SCC154 cells after treatment with 26.01μM CdCl 2 (12h). ACTIN and DAPI dyes were used to detect microfilament and nuclei, respectively. Scale bar: 20 μM. All data represent the mean of three independent experiments. Histograms are reported as mean ± SD. p -value: *≤0.05; ***≤0.001; ****≤0.0001.

Journal: International Journal of Biological Sciences

Article Title: Acute Exposure to Cadmium Triggers NCOA4-Mediated Ferritinophagy and Ferroptosis in Never-Smokers Oral Cancer Cells

doi: 10.7150/ijbs.111228

Figure Lengend Snippet: CAL27 cells shows lipid peroxidation after CdCl 2 administration. (A) Flow cytometry analysis and relative histograms of lipid peroxidation quantified by using BODIPY-C11 in CAL27 and SCC154 cells upon treatment with 26.01μM CdCl 2 (12h). (B) Western blot analysis and relative optical densitometry of GPX4 in CAL27 and SCC154 cells treated with 26.01μM CdCl 2 (12h). GAPDH was used as normalization control for protein quantification. (C) Fluorescence microscopy analysis of GSH content in CAL27 and SCC154 cells after treatment with 26.01μM CdCl 2 (12h). ACTIN and DAPI dyes were used to detect microfilament and nuclei, respectively. Scale bar: 20 μM. All data represent the mean of three independent experiments. Histograms are reported as mean ± SD. p -value: *≤0.05; ***≤0.001; ****≤0.0001.

Article Snippet: Human oral squamous cell lines (OSCC) - CAL27, OT1109, SCC090, and SCC154 - were purchased from the American Type Culture Collection (ATCC, Rockville, MD, United States).

Techniques: Flow Cytometry, Western Blot, Control, Fluorescence, Microscopy

CdCl 2 citotoxicity is associated with the ability of cadmium to compete with iron. (A-B). Quantification of iron and cadmium intracellular amount through ICP-MS in CAL27 and SCC154 cells treated with 26.01μM CdCl 2 at 30', 1h, 6h, and 12h. (C-D) . ICP-MS analysis of iron and cadmium intracellular amount in CAL27 and SCC154 cells upon treatment with 26.01μM CdCl 2 (12h) alone or in combination with ferlixit (25, 50 and 100μM for 12h). (E) PI flow cytometry assay and relative histograms of CAL27 and SCC154 cells treated with CdCl 2 (26.01μM for 12h) alone or in combination with ferlixit (25, 50 and 100 μM for 12h). % of dead cells (PI positive) are reported in each dot plot. (F) Western blot analysis and relative optical densitometry of NCOA4 and FtH1 in CAL27 and SCC154 cells treated with 26.01μM CdCl 2 (12h) alone or in combination with 100μM ferlixit (12h). GAPDH was used as normalization control for protein quantification. All data represent the mean of three independent experiments. Histograms are reported as mean ± SD. p -value: *≤0.05; **≤0.01; ***≤0.001; ****≤0.0001. ns: not significant.

Journal: International Journal of Biological Sciences

Article Title: Acute Exposure to Cadmium Triggers NCOA4-Mediated Ferritinophagy and Ferroptosis in Never-Smokers Oral Cancer Cells

doi: 10.7150/ijbs.111228

Figure Lengend Snippet: CdCl 2 citotoxicity is associated with the ability of cadmium to compete with iron. (A-B). Quantification of iron and cadmium intracellular amount through ICP-MS in CAL27 and SCC154 cells treated with 26.01μM CdCl 2 at 30', 1h, 6h, and 12h. (C-D) . ICP-MS analysis of iron and cadmium intracellular amount in CAL27 and SCC154 cells upon treatment with 26.01μM CdCl 2 (12h) alone or in combination with ferlixit (25, 50 and 100μM for 12h). (E) PI flow cytometry assay and relative histograms of CAL27 and SCC154 cells treated with CdCl 2 (26.01μM for 12h) alone or in combination with ferlixit (25, 50 and 100 μM for 12h). % of dead cells (PI positive) are reported in each dot plot. (F) Western blot analysis and relative optical densitometry of NCOA4 and FtH1 in CAL27 and SCC154 cells treated with 26.01μM CdCl 2 (12h) alone or in combination with 100μM ferlixit (12h). GAPDH was used as normalization control for protein quantification. All data represent the mean of three independent experiments. Histograms are reported as mean ± SD. p -value: *≤0.05; **≤0.01; ***≤0.001; ****≤0.0001. ns: not significant.

Article Snippet: Human oral squamous cell lines (OSCC) - CAL27, OT1109, SCC090, and SCC154 - were purchased from the American Type Culture Collection (ATCC, Rockville, MD, United States).

Techniques: Flow Cytometry, Western Blot, Control

Proteomic analysis of OSCC cells treated with CdCl 2 . (A) Heatmap of DEPs (TOP 20 up and TOP 20 down) in CAL27 cells treated with 26.01μM CdCl 2 (12h) vs untreated. Color intensity is proportional to the magnitude of changes. Relative expression levels are shown in red (upregulation) and green (downregulation). (B) Heatmap of DEPs in SCC154 cells treated with 26.01μM CdCl 2 (12h) vs untreated. Color intensity is proportional to the magnitude of changes. Relative expression levels are shown in red (upregulation) and green (downregulation). (C) GO analysis of of DEPs in CAL27 cells treated with 26.01μM CdCl 2 (12h) vs untreated. The dot size denotes the number of DEPs, while colors correspond to the adjusted p -value range. (D) p-ƔH2AX flow cytometry analysis and relative histograms CAL27 and SCC154 cells treated with CdCl 2 (26.01μM for 12h). % of positive ( + ) cells are reported in each dot plot. Each experiment was performed in triplicate. Histograms are presented as mean ± SD. p -value: ****≤0.0001. ns: not significant.

Journal: International Journal of Biological Sciences

Article Title: Acute Exposure to Cadmium Triggers NCOA4-Mediated Ferritinophagy and Ferroptosis in Never-Smokers Oral Cancer Cells

doi: 10.7150/ijbs.111228

Figure Lengend Snippet: Proteomic analysis of OSCC cells treated with CdCl 2 . (A) Heatmap of DEPs (TOP 20 up and TOP 20 down) in CAL27 cells treated with 26.01μM CdCl 2 (12h) vs untreated. Color intensity is proportional to the magnitude of changes. Relative expression levels are shown in red (upregulation) and green (downregulation). (B) Heatmap of DEPs in SCC154 cells treated with 26.01μM CdCl 2 (12h) vs untreated. Color intensity is proportional to the magnitude of changes. Relative expression levels are shown in red (upregulation) and green (downregulation). (C) GO analysis of of DEPs in CAL27 cells treated with 26.01μM CdCl 2 (12h) vs untreated. The dot size denotes the number of DEPs, while colors correspond to the adjusted p -value range. (D) p-ƔH2AX flow cytometry analysis and relative histograms CAL27 and SCC154 cells treated with CdCl 2 (26.01μM for 12h). % of positive ( + ) cells are reported in each dot plot. Each experiment was performed in triplicate. Histograms are presented as mean ± SD. p -value: ****≤0.0001. ns: not significant.

Article Snippet: Human oral squamous cell lines (OSCC) - CAL27, OT1109, SCC090, and SCC154 - were purchased from the American Type Culture Collection (ATCC, Rockville, MD, United States).

Techniques: Expressing, Flow Cytometry

CdCl 2 exposure induces G2-M phase arrest in CAL27 cells derived from never smokers. Cell cycle analysis via flow cytometry and relative histograms of CAL27 (A) and SCC154 (B) cells treated with 26.01μM CdCl 2 for 1h and 12h. Each experiment was performed in triplicate. Histograms are presented as mean ± SD. p -value: *≤0.05; **≤0.01. ns: not significant.

Journal: International Journal of Biological Sciences

Article Title: Acute Exposure to Cadmium Triggers NCOA4-Mediated Ferritinophagy and Ferroptosis in Never-Smokers Oral Cancer Cells

doi: 10.7150/ijbs.111228

Figure Lengend Snippet: CdCl 2 exposure induces G2-M phase arrest in CAL27 cells derived from never smokers. Cell cycle analysis via flow cytometry and relative histograms of CAL27 (A) and SCC154 (B) cells treated with 26.01μM CdCl 2 for 1h and 12h. Each experiment was performed in triplicate. Histograms are presented as mean ± SD. p -value: *≤0.05; **≤0.01. ns: not significant.

Article Snippet: Human oral squamous cell lines (OSCC) - CAL27, OT1109, SCC090, and SCC154 - were purchased from the American Type Culture Collection (ATCC, Rockville, MD, United States).

Techniques: Derivative Assay, Cell Cycle Assay, Flow Cytometry

Effects of CdCl 2 exposure in CAL27T cells. To obtain CAL27T (CAL27 Tolerant to Cd) CAL27 were exposed to low doses of CdCl 2 (10μM) for 30 days. (A) PI flow cytometry assay and relative histograms of CAL27T cells treated with CdCl 2 (26.01μM for 12h). % of dead cells (PI positive) are reported in each dot plot. (B) Quantification of iron and cadmium intracellular amount through ICP-MS in CAL27T cells treated with 26.01μM CdCl 2 (12h). (C) Western blot analysis and relative optical densitometry of NCOA4, FtH1, LC3B (I-II), mTORC1 and p-mTORC1 in CAL27T cells treated with 26.01μM CdCl 2 (12h). GAPDH was used as normalization control for protein quantification. (D) Fluorescence microscopy analysis of LIP content with FerroOrange dye in CAL27T cells after treatment with 26.01μM CdCl 2 (12h). Flow cytometry analyses and relative histograms of mitochondrial ROS amount (E) , mitochondrial membrane potential (F) and lipid peroxidation (G) assessed by using MitoSOX, TMRM and BODIPY-C11 reagents, respectively, in CAL27T cells following treatment with 26.01μM CdCl 2 (12h). Each experiment was performed in triplicate. Histograms are presented as mean ± SD. p -value: *≤0.05; ***≤0.001; ****≤0.0001. ns: not significant.

Journal: International Journal of Biological Sciences

Article Title: Acute Exposure to Cadmium Triggers NCOA4-Mediated Ferritinophagy and Ferroptosis in Never-Smokers Oral Cancer Cells

doi: 10.7150/ijbs.111228

Figure Lengend Snippet: Effects of CdCl 2 exposure in CAL27T cells. To obtain CAL27T (CAL27 Tolerant to Cd) CAL27 were exposed to low doses of CdCl 2 (10μM) for 30 days. (A) PI flow cytometry assay and relative histograms of CAL27T cells treated with CdCl 2 (26.01μM for 12h). % of dead cells (PI positive) are reported in each dot plot. (B) Quantification of iron and cadmium intracellular amount through ICP-MS in CAL27T cells treated with 26.01μM CdCl 2 (12h). (C) Western blot analysis and relative optical densitometry of NCOA4, FtH1, LC3B (I-II), mTORC1 and p-mTORC1 in CAL27T cells treated with 26.01μM CdCl 2 (12h). GAPDH was used as normalization control for protein quantification. (D) Fluorescence microscopy analysis of LIP content with FerroOrange dye in CAL27T cells after treatment with 26.01μM CdCl 2 (12h). Flow cytometry analyses and relative histograms of mitochondrial ROS amount (E) , mitochondrial membrane potential (F) and lipid peroxidation (G) assessed by using MitoSOX, TMRM and BODIPY-C11 reagents, respectively, in CAL27T cells following treatment with 26.01μM CdCl 2 (12h). Each experiment was performed in triplicate. Histograms are presented as mean ± SD. p -value: *≤0.05; ***≤0.001; ****≤0.0001. ns: not significant.

Article Snippet: Human oral squamous cell lines (OSCC) - CAL27, OT1109, SCC090, and SCC154 - were purchased from the American Type Culture Collection (ATCC, Rockville, MD, United States).

Techniques: Flow Cytometry, Western Blot, Control, Fluorescence, Microscopy, Membrane

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